DNA Extraction of Almond without Phenol and Liquid Nitrogen

DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol

Mangroves and salt marsh species are known to synthesize a wide spectrum of polysaccharides and polyphenols including flavonoids and other secondary metabolites which interfere with the extraction of pure genomic DNA. Although a plethora of plant DNA isolation protocols exist, extracting DNA from mangroves and salt marsh species is a challenging task. This study describes a rapid and reliable c...

Short Communication: DNA Extraction Using Liquid Nitrogen in Staphylococcus aureus

Short Communication: DNA Extraction Using Liquid Nitrogen in Staphylococcus aureus

Fast protocol for extraction of DNA from Prosopis spp leaves (plant adapted to arid environment) without liquid nitrogen.

The extraction of high-quality genomic DNA from Prosopis spp for polymerase chain reaction (PCR) amplification is complicated, owing to the presence of a high percentage of secondary metabolites that bind to or co-precipitate with nucleic acids. In the present study, we report a modified sodium dodecyl sulfate/phenol protocol that eliminates the use of liquid nitrogen in the maceration process,...

Biological Values of Wheat and Almond Nitrogen

The attempt to compare the biological values of various protein foods, made by Karl Thomas (1) in 1909, has resulted in a good deal of discussion, but few confirming experiments. The brevity of his nitrogen balance periods, as well as the arbitrary formulas used by him in the calculation of the percentage efficiencies reported, are the basis of criticism made recently by American authors (2, 3)...

Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation†